【德国IBIDI】μ-Slide ibiPore Transwell细胞侵袭－85016 85026

Applications:

1.Trans-endothelial migration under flow conditions

2.Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix

3.Apical-basal cell polarity assays

4.Skin and lung models with liquid-air interface

5.Cell barrier model assays with apical-basal gradients

6.Cell migration assays based on filters and porous membranes

Technical Features:

1.SiMPore’s G-FLAT™ Microporous Glass Membranes

2.Cross-channel structure with a porous optical membrane in between

3.Excellent optical properties, comparable to a coverslip

4.Pore sizes 0.5 μm, 3 μm, 5 μm, or 8 μm

5.Membrane thickness 0.3 μm (300 nm) 

6.For use with objective lenses with a working distance >0.5 mm 

7.Fully compatible with the ibidi Pump System 

8.Defined shear stress and shear rate levels 
(For details see Application Note 11)

Endothelial cells on 3 μm ibiPore membrane. Immunofluorescence overlay image of phase contrast, DAPI (blue), VE-Cadherins (green), F-Actin (red).

Fibroblast cells on 3 μm ibiPore membrane. 
Phase contrast image, objective lens 4x.

Recommendations for Applications: Pore Size & Porosity

The bigger the cell – the bigger the recommended pore size. 

Recommended Pore Sizes for Different Applications

Cross Section of the μ-Slide Membrane ibiPore Flow

Composition of the μ-Slide Membrane ibiPore Flow

3D View with Cross-Channel Structure and Central ibiPore Membrane

Application Examples

SEM Images of the Porous Glass Membrane

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